›› 2011, Vol. 23 ›› Issue (5): 335-339.doi: 10.3969/j.issn.1004-616x.2011.05.003

• 论著 • Previous Articles     Next Articles

Linkage analysis between 8 STR markers of chromosome 12 and Kashin-Beck disease in nuclear families

GAO Xiao-yun，KANG Lei，YU Min，ZHOU Yu-ling，GUO Xiong*   

  1. 1. Department of Immunology, Medical College, Xi’an Jiaotong University, Xi’an 710061; 2. Faculty of Public Health, Key Laboratory of Environment and Diseases Related Genes of Ministry of Education, Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China
  • Received:2010-11-09 Revised:2011-05-26 Online:2011-09-30 Published:2011-09-30
  • Contact: GUO Xiong

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Abstract: OBJECTIVE: To analyze linkage between 8 short tandem repeat (STR) markers of chromosome 12 and Kashin-Beck disease (KBD)in nuclear families. METHODS：The probands in 23 nuclear families and their 90 members diagnosed by national clinical criteria to diagnose KBD，and their blood samples were collected. The types of the 8 STR locus (D12S1613，D12S1725，D12S1663，D12S1697，D12S1675，D12S358，D12S1638 and D12S1682) polymorphisms were measured by gene scan，and gene frequencies and linkage analyses calculated between these polymorphisms and KBD. RESULTS：11，10，5，5，10，8，7 and 11 alleles on 8 STR locus (D12S1613， D12S1725，D12S1663，D12S1697，D12S1675，D12S358，D12S1638 and D12S1682) of chromosome 12 were detected，and the LOD scores of D12S1725 was 2.52. CONCLUSION：The susceptable gene linkage locus of KBD may be close to maker D12S1725.

Key words: Kashin-Beck disease, gene polymorphism, linkage